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PARTIAL CHARACTERIZATION OF BACTERIOCIN LIKE INHIBITORY SUBSTANCE FROM BACILLUS SUBTILIS BS15, A LOCAL SOIL ISOLATE

SYED IQBAL ALAM¹, MUHAMMAD KAMRAN¹, MUHAMMAD SOHAIL², AQEEL AHMAD² AND SHAKEEL AHMED KHAN²

Abstract: The aim of this study was to investigate the production of bacteriocin/bacteriocin-like inhibitory substances (BLIS) from Bacillus subtilis BS15, isolated from soil. The inhibitory substance was partially purified and characterized as BLIS with a molecular-weight of 3-5 kDa, as determined by SDS-PAGE. Its production was observed during the late exponential-phase or at the beginning of stationary-phase. It retained its activity up to 80°C and over a wide range of pH i.e., 3-9. It was found active against several clinically important bacterial species such as Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Salmonella typhi and also against the food-spoilage causing microbes, and may be considered as future food preservative. 

¹Department of Microbiology, Federal Urdu University of Arts, Science and Technology Karachi, 

²Department of Microbiology, University of Karachi, Karachi-75270 Pakistan