|
|
|
OPTIMIZATION OF CULTURAL CONDITIONS FOR THE BIOSYNTHESIS OF
LIPASES BY PENICILLIUM CHRYSOGENUM (MBL 22) THROUGH
SOLID STATE FERMENTATION
TEHREEMA IFTIKHAR*1, MUBASHIR NIAZ1,
ZAIB UN NISA1, ASMA TARIQ1, M.N.KHALID1 AND RUKHSANA JABEEN2
Abstract: The present study was conducted in the Laboratory of
Biotechnology and Mycology, Department of Botany, GC University,
Faisalabad with the objective to study the production of extracellular
lipases by Penicillium chrysogenum through solid- state
fermentation (SSF). Penicillium chrysogenum was screened for the
extracellular lipase production. The maximum extracellular lipase
activity was obtained at 28oC after 72 h by using 1 mL
inoculum level. 15g of mixed substrates (Almond+ Soybean+ Sunflower) was
optimized for maximum extracellular lipase production. Agricultural by
products, nitrogen sources and carbon sources were also optimized for
the maximum production of enzyme. Maximum production of the
extracellular lipase (64.77±0.155a U/mL) was obtained when
olive oil was used as additional oil. Tween 80 and yeast extract were
optimized as the best additional carbon and nitrogen sources
respectively.
1Laboratory
of Mycology & Biotechnology, Department of Botany, Government College
University Faisalabad, Pakistan
2Department
of Plant Sciences SBKW University, Quetta, Pakistan
|
|
|