|
|
|
PROCESS OPTIMIZATION FOR A POTENT WILD AND MUTANT STRAIN OF
ASPERGILLUS NIGER FOR BIOSYNTHESIS OF AMYLOGLUCOSIDSAE
SHAZIA MALIK1,
TEHREEMA IFTIKHAR2*AND IKRAM UL HAQ1
Abstract:
The present study is
concerned with the selection of a potent strain of Aspergillus niger
and optimization of the cultural conditions for the biosynthesis of
amyloglucosidase. The cultural conditions were optimized for the enzyme
production. Twenty percent (50/250ml flask) was found to be optimum
volume of the medium. Optimum temperature was 30oC after 72 h
of incubation, with the initial pH of the medium 5.0. 2% Starch with 1%
glucose as an additional carbon source gave maximum amyloglucosidase
production Addition of 0.3% ammonium sulphate in the fermentation medium
increased the enzyme production while 2% spore inoculum showed best
amyloglucosidase production.
1Institute of Industrial Biotechnology, GC University, Lahore
2Department of Botany, GC University, Faisalabad
*Corresponding author E-mail:
pakaim2001@yahoo.com
|
|
|