|
|
|
MOLECULAR MARKERS
ASSISTED GENETIC CHARACTERIZATION OF SOME SELECTED
WILD POACEAE SPECIES
AYESHA ZEB1,
ZABTA KHAN SHINWARI2 AND TARIQ MAHMOOD1*
Abstract:
Genetic
diversity of ten selected wild species belonging to family Poaceae
was assessed by using RAPD markers. The species consisted of Tragus
roxburgii, Eragrostis poaeoides, Brachiaria distachya,
Dactyloctenium aegyptium, Setaria glauca, Setaria
verticillata, Chrysopogon aucheri, Heteropogon
contortus, Saccharum spontaneum and Themeda
anathera. In total 10 primers of OPC series were used, of which 9
primers gave reproducible amplifications. Out of 139 bands produced, 92
bands were shown to be polymorphic leading to 66% polymorphism across
the species. NTSYS cluster analysis showed 59% similarity among the
selected species indicating their common phylogenetic origin and more
genetic stability during evolution. The genetic similarity index value
ranged from 0.51 to 0.89. The results indicated high level of genetic
diversity among grass species on the basis of RAPD technique. There is
need to study other members of family Poaceae from different
populations by using other molecular markers.
1Department of Plant Sciences, Quaid-i-Azam University, Islamabad-46320,
Pakistan
2Department of Biotechnology, Quaid-i-Azam University, Islamabad-46320,
Pakistan
|
|
|