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Pak. J. Bot., 43(5): 2295-2300, 2011. |
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Updated: 18-10-11 | ||||
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COMPARATIVE ASSESSMENT OF GENETIC VARIABILITY IN CRYPTOLEPIS BUCHANANII, TYLOPHORA HIRSUTA AND WATTAKAKA VOLUBILIS
TARIQ MAHMOOD1*, ADEEBA TARIQ1,
NAZIA NAZAR1, BILAL HAIDER ABBASI2 AND S. M. SAQLAN NAQVI3
Abstract:
Asclepiadaceae is an economically important family with great medicinal
value. However, very little work has been carried out on the genetic
variability of Asclepiadaceae members especially on some medicinally
important species like Tylophora hirsuta, Wattakaka volubilis
and Cryptolepis buchananii. Keeping in view the importance of
these species, a study was designed to explore the genetic diversity of
these 3 species of Asclepiadaceae and the plant material was collected
from Quaid-i-Azam university campus, Islamabad. To assess the genetic
variability and polymorphism among these species, randomly amplified
polymorphic DNA (RAPD) markers were used. Sixty RAPD primers from OPA,
OPC, OPF and OPG series were used; only 8 primers of OPC series gave
amplification. Maximum polymorphism at interspecific and intraspecific
levels was shown by OPC9 and minimum polymorphism was observed in OPC5.
The data was analyzed using NTSYS software pc version 2.02. Low genetic
diversification was observed at intraspecific and interspecific level.
Moreover, during cluster analysis Tylophora hirsuta and
Wattakaka volubilis were found to be present in the same cluster
showing a close relationship whereas Cryptolepis buchananii
appeared in a separate cluster.
1Plant
Biochemistry and Molecular Biology Laboratory, Department of Plant
Sciences,
Quaid-i-Azam University, Islamabad-45320,
Pakistan |
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