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Pak. J. Bot., 43(5): 2415-2418, 2011.

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CALLUS INDUCTION AND PLANT REGENERATION FROM LEAF EXPLANT OF CORNUKAEMPFERIA AURANTIFLORA MOOD & LARSEN

PIYAPORN SAENSOUK

Abstract: Young leaves of Cornukaempferia aurantiflora Mood & Larsen were used for the initiation of callus growth on Murashige Skoog medium supplemented with 3% sucrose and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in the light. The highest number of callus forming, percentage of callus formation and average weight of callus were obtained from young leaves cultured on the medium supplemented with 2.0 mg/l 2,4-D. Shoots were successfully regenerated on the medium with concentrations of 2,4-D and BA added.

Department of Biology, Faculty of Science, Mahasarakham University, Khamriang, Kantarawichai, 41150, Thailand.