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IN VITRO
MUTAGENESIS IN BANANA AND VARIANT SCREENING THROUGH ISSR
ABDULLAH KHATRI1,
SAJIDA BIBI1, MAHUMMAD UMAR DAHOT2, IMTIAZ AHMED KHAN1
AND GHULAM SHAH NIZAMANI1
Abstract:
An efficient culture
medium for In vitro clonal propagation of banana cultivars was
established. Meristamtic tips of Basrai and GCTCV-215 were irradiated
with different doses of gamma rays viz., 0, 10, 20, 30 and 40Gy through
cobalt 60 source. Eleven random ISSR primers were used to study genetic
variation among the radiated population. ISSR primers produced 37 bands
in all the clones studied. Two monomorphic and 35 polymorphic bands were
observed during the study.
The
number of fragments produced by the primers ranged from 2-5, with an
average of 3.45 fragments. Fragments size ranged from 198bp-1.842kb.
Genetically most
similar genotypes were G-215MP3 and G-215MP4 (89.8%) while most
dissimilar genotypes were Basrai and G-215MP2 (38.5%). On the basis of
similarity matrix clones could be divided into three clusters and two
groups.
1Nuclear Institute of Agriculture, Tando Jam, Pakistan
2Institute of Genetic Engineering and Biotechnology, University of Sindh,
Jamshoro, Pakistan
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