|
|
|
PROBING GENETIC
DIVERSITY TO CHARACTERIZE RED ROT RESISTANCE IN
SUGARCANE
ABDUL SAMAD MUMTAZ1*,
DUR-E-NAYAB1, MUHAMMAD JAVED IQBAL2 AND ZABTA KHAN SHINWARI3
Abstract:
Genetic
diversity was assessed in a set of twelve sugarcane genotypes using
Random Amplified Polymorphic DNA (RAPD). A total of thirty-two
oligo-primers were employed, sixteen of them revealed amplification at
149 loci, out of which 136 were polymorphic. The genotype SPSG-26 showed
the highest number of polymorphic loci, followed by CSSG-668 and
HSF-242. Pairwise genetic similarity ranged from 67.2% to 83.3%. The
UPGMA cluster analysis resolved most of the accessions in two groups.
The clustering pattern did not place all resistant varieties in one or
related group which depict diverse resistance source in the present set
of sugarcane varieties. Ten primers revealed genotype specific bands
among which four primers (K07, H02, K10 and F01) produced multiple
genotype specific bands that aid genotype identification especially
those with red rot resistance. The present study not only provided
information on the genetic diversity among the genotypes but also
revealed the potential of RAPD-PCR markers for genotype identification
and therefore could be utilized in marker assisted selection for red rot
resistance in sugarcane.
1Department of Plant Sciences, Quaid-i-Azam University, Islamabad,
Pakistan
2School of Biological Sciences, University of the Punjab Lahore, Pakistan
3Department of Biotechnology and Bioinformatics, Quaid-i-Azam University,
Islamabad, Pakistan
*Corresponding author:
asmumtaz@qau.edu.pk
|
|
|