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BIOSYNTHESIS OF
EXTRACELLULAR LIPASES BY BACILLUS SP. (MBLB-3) IN RELATION TO THE
NUTRITIONAL CONDITIONS
TEHREEMA IFTIKHAR*1,
MUBASHIR NIAZ1, M. ANJUM ZIA2, RUKHSANA JABEEN3
AND MUHAMMAD NAUMAN AFTAB4
Abstract:
The present study deals
with the microbial biosynthesis of lipases by a strain of Bacillus
sp. Fifteen strains were isolated from oily products. These strains were
screened for the production of lipases by solid state fermentation in
250mL Erlenmeyer flasks. Of all the strains examined, MBLB-3 gave
maximum production (20.4 ± 0.2a U/g) of lipases. Different
agricultural by-products such as wheat bran, rice husk, almond meal,
cotton seed meal, soybean meal, sunflower meal and mustard meal were
used as substrates. Maximum extracellular lipase activity (33.9 ± 0.5a
U/g) was observed when almond meal was used as the substrate and it was
moistened with phosphate buffer (pH.8.0). The reaction was carried out
by taking bacterial cells as a source of lipases in solid substrate
fermentation. 0.5 % of Tween 80 was optimized for the maximum production
(42.58 ± 0.8a U/g) of lipases. The above results revealed
that the Bacillus lipase can be a good additive to be used in
detergents.
1Laboratory of Mycology & Biotechnology, Department of Botany, Govt.
College University, Faisalabad
2Laboratory of Biotechnology, Department of Chemistry & Biochemistr,
University of Agriculture, Faisalabad
3Department of Plant Sciences SBKW University, Quetta, Pakistan
4Institute of Industrial Biotechnology, Govt. College University, Lahore
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