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INFLUENCE OF HERBAL
DYE EXTRACTED FROM DRY WOOD OF INDIGENOUS BERBERIS PACHYACANTHA
KOCHNE IN PLANT HISTOLOGICAL STAINING
HIKMAT ULLAH JAN1,
ZABTA KHAN SHINWARI*1 AND KHAN BAHADER MARWAT2
Abstract:
The staining
capacity of Berberis pachyacantha Kochne extract on histological
sections of angiosperms stem was determined. A yellow pigment extracted
from the powder of dried stem of B. Pachyacantha with different
solvents. Staining dye was chemically extracted from the dry wood of
Berberis pachyacantha Kochne in solvents like clove oil, ethanol,
water, and its influence as histological staining agent for angiospermic
plants was studied. The dye in 10% w/v clove oil was found more
effective, showing high intensity to stain stem tissue of monocots (
Zea mays L.) section, while the dye extracted from wood of
Berberis pachyacantha Kochne in 10% w/v ethanol was found
highly effective and showing high intensity to stain stem tissue of
dicot using Helianthus annuus L. stem cross section. Staining
power of the extracted dye of Berberis pachyacantha Kochne
with respect of different stem tissue was actually variable according to
the solvent used, but they proved to be weak staining agent for
parenchyma of both monocot and dicot stem tissue. The recognition of
dynamic ingredients of dye will open a new way of research in the field
of dyeing. Current research work would also be helpful to explore better
solvents for the means of dye extraction. In the dye extracts further
research on the analysis of the active chemical substances, improvement
of colour imparting, the extracts shelf life, attribute through the
preamble of mordant into the dye extract is convenient. Present
breakthrough will go a long way in minimizing over-dependence on toxic,
as well as synthetic, expensive and non available exotic stains in the
future.
1Department of Biotechnology, Faculty of Biological Sciences,
Quaid-i-Azam University,
Islamabad, Pakistan.
2Agriculture University of Peshawar, Khyber Pakhtunkhwa, Pakistan.
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