|
|
IN VITRO
BACTERICIDAL AND BACTERIOSTATIC POTENTIAL OF INGRIDIENTS OF
TRADITIONAL MEDICINE OBTAINED FROM KACHA AREA (RIVER INDUS) DISTRICT
D.I.KHAN, KPK, AGAINST HUMAN BACTERIAL PATHOGENS
ADNAN AMIN* AND M. AYAZ KHAN
Abstract: The aim of this study
was to analyze and evaluate antimicrobial potential of medicinal
plants obtained from kacha area of river indus, that are used as
ingredients of traditional medicine for treatment of multiple
infectious diseases. The antimicrobial activities of methanol and
aqueous extracts of 5 medicinal plants of a traditional medicine were
evaluated against 6 human gram positive (Staphylococcus aureus,
Micrococcos luteus) and gram negative (Escherichia coli,
Pseudomonas aeruginosa, Enterobacter, Klebsiella pneumoniae)
pathogens. The disc diffusion and broth macro dilution assay was used
to determine the zone of inhibitions and the minimum inhibitory
concentration respectively. The ciprofloxacin and streptomycin were
used as standard agents. Both aqueous and methanol fractions of all 5
tested plants exhibited antimicrobial activity against one or more
species of microorganisms. The most active extract found was
Azadirachta indica leaves which represented widest zone of
inhibition of 16(±0.05) mm and minimum inhibitory concentration
0.19mg/ml against Klebsiella pneumoniae.
Calotropis procera leaves was found least active representing
lowest Zones of inhibition 3.13(±0.05)
mm
and highest minimum inhibitory concentration value (20mg/ml) against
test microorganisms. Over all methanol fractions of medicinal plants
represented stronger biological activity against test microorganisms
than aqueous extracts. A good majority of extracts were bactericidal.
These results afford the ground information for potential use of crude
extracts with high MIC and MBC values. Moreover a synergistic effect
is expected when used in combination. For this further attempt are in
progress to investigate antimicrobial potential of combination
medicine.
Gomal Center of Biochemistry and Biotechnology (GCBB),
Gomal University, D.I. Khan,KPK, Pakistan.
|
|
|