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IN VITRO
EVALUATION OF ANTHELMINTIC ACTIVITY OF ESSENTIAL OILS FROM DIFFERENT
PARTS OF
SKIMMIA LAUREOLA (DC.) ZUCC. EX WALP., VER. NAIR
FERHAT MEHMOOD*,
MUHAMMAD QASIM1, ZAHEER-UD-DIN KHAN, NAEEM IQBAL1, SHAHID MEHMOOD2,
MUHAMMAD LATEEF3 AND PHOOL SHAHZADI2
Abstract:
In this study ‘anthelmintic
activity of essential oils (EOs), of leaf, stem and root of Skimmia
laureola (DC.) Zucc. Ex Walp., ver. Nair was evaluated. ‘Adult
motility assay’ was employed using Haemoncus contortus adult
worms. EO was applied at three levels viz. 10, 35 and 50µL/10 ml in
phosphate buffer solution (PBS) plus 10µL of tween 20 (as
carrier/emulsifier). Levamisole was used as a positive control at 0.55
mg/ml concentration. Each concentration of essential oils obtained from
different plant parts exhibited varied anthelmintic activity.
The best dose dependant effect on adult mortality was
by
EOs from
S. laureola
root and stem (R2
values 0.801). Change in the color of the dead worms was an
indicator parameter of the fact that the EOs might have damaged the skin
of the worms or after transcutaneous penetration into the body has
disrupted the circulatory system by causing constriction of blood
vessels. It is concluded that EOs extracted from Skimmia laureola
have anthelmintic properties.
Department of
Botany, G.C. University, Lahore, Pakistan,
1Department
of Botany, G.C. University, Faisalabad Pakistan,
2P.C.S.I.R.
Labs, Lahore, Pakistan,
3University of Veterinary and Animal
Sciences Lahore, Pakistan.
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