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In vitro
growth of
Melaleuca alternifolia
Cheel IN bioreactor of
immersion by bubbles
Gessiel
Newton Scheidt1, André Luís Lopes da Silva2,
Yohana de Oliveira2,
Jefferson da Luz Costa2, Luiz Antonio Biasi2 AND
Carlos Ricardo Soccol2*
Abstract:
Development of
new techniques for in vitro culture is necessary to optimize and
reduce the production costs. The aim of this work was to introduce the
bioreactor BIB® (Bioreactor of Immersion by Bubbles) and
compare it with conventional culture and bioreactor RITA®
(Recipient with Automatized Temporary Immersion) for In vitro
growth of M. alternifolia plantlets. Plantlets with 1.0 cm height
from the fifth In vitro subculture were used. Treatments were:
(1) conventional culture (control) using 200 mL glass flasks covered
with polypropylene lids; (2) bioreactor RITA® and (3)
bioreactor BIB®. Continuous immersion and aeration was used
in bioreactors. It was used liquid MS medium supplemented with 30 g.L-1
sucrose and 1 drop.200 mL-1 Tween 20® (i.e.
detergent for bubbles formation). BIB® obtained best results
for total fresh mass (0.39 g) and shoot fresh mass (0.27 g), fresh mass
increment (3.1), total dry mass (0.036 g) and shoot dry mass (0.033 g)
than RITA® and conventional culture. In conclusion, BIB®
is more efficient to the biomass accumulation of M.
alternifolia plantlets.
1Federal
University of Tocantins, Campus Gurupi, Brazil
2Federal
University of Paraná, Curitiba, Brazil
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