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ASSESSMENT OF
ANTIBACTERIAL ACTIVITY OF IN VITRO AND IN VIVO
GROWN GARLIC (ALLIUM
SATIVUM L.)
ANEELA FATIMA,*
TAUQEER AHMAD, SHAISTA J. KHAN, FARAH DEEBA AND NASREEN ZAIDI
Abstract:
Antibacterial
activities of In vitro and In vivo grown garlic were
compared against five bacterial strains Escherichia coli,
Klebsiella pneumoniae, Proteus mirabilis, Enterobacter
aerogenes and Staphylococcus aureus. In 8-9 weeks, In
vitro garlic bulblets were produced from shoot tip explants callus
of garlic cloves cultured on modified MS medium. Ten µM BA induced
somatic embryogenesis in soft, granular and dirty white to yellow
colored clumps of calli, regenerated into plantlets, which eventually
transformed into 9 or 10 mm dia bulblets on basal MS medium. Clear zones
of inhibition were demarked by paper disc diffusion method. The content
of micro-bulblets expressed greater antimicrobial activity than that of
In vivo garlic cloves through wider diameter zone of inhibition
against said bacterial strains. In vitro garlic extract formed
24mm and 22mm zones being the widest zones against Klebsiella and
Proteus respectively.
Food and Biotechnology
Department, PCSIR Laboratories Complex, Ferozepur Road Lahore-54600,
Pakistan.
*Corresponding author. E-mail:
aneela.fm@gmail.com Phone No. +92429230688-95, Ext. 288
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