Pak. J. Bot., 43(SI): 169-174, December, 2011 | Back to Contents | ||||
|
Updated: 03-12-11 | ||||
|
ANTIMICROBIAL POTENTIALS OF ECLIPTA ALBA BY WELL DIFFUSION METHOD
JEHAN BAKHT1*, AMJAD ISLAM1 AND MOHAMMAD SHAFI2
Abstract:
The
susceptibility of nine microbial species to an antimicrobial extract
from Eclipta alba was screened using the well diffusion assay.
Three different volumes (24, 30 and 36 µl/well) were tested. Analysis of
the data revealed that all extracts from Eclipta alba showed
antimicrobial activities. An N-butanol fraction showed inhibitory
activities against all nine microbial species. Samples extracted with
petroleum ether, dichloromethane, methanol or water had varying levels
of inhibition against some of these microorganisms. The most resistant
microbial strain was Salmonella typhi when tested against
petroleum ether, dichloromethane, methanol and water extracted samples.
The most susceptible Gram-positive bacterium was Bacillus subtilis,
which was inhibited by all six extracts from Eclipta alba, while
the most resistant Gram-positive bacterium was Bacillus cereus.
Erwinia carotovora was the most susceptible Gram-negative
bacterium, while Salmonella typhi and Escherichia coli
were highly resistant among the Gram-negative bacteria. 1Institute of Biotechnology and Genetic Engineering KPK Agricultural University Peshawar Pakistan 2Department of Agronomy KPK Agricultural University Peshawar Pakistan |
||||
|
|||||
Back to Contents |
|
Back to Contents | |||
|