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MOLECULAR ANALYSES OF GLADIOLUS LINES WITH IMPROVED RESISTANCE AGAINST FUSARIUM WILT

IDREES AHMAD NASIR^1*, ARSHAD JAMAL¹, ZIAUR RAHMAN¹ AND TAYYAB HUSNAIN¹

Abstract: The genetic changes/variations taken place during the process of in-vitro selection for improvement of Fusarium resistance in the susceptible Gladiolus cultivar were assessed.  The selected cell lines showing resistance against Fusarium oxysporum were analyzed with total of 29 amplified reproducible RAPD fragments.  The number of fragments per primer ranged from 4 in S13 and R8 to 10 fragments in S19.  The total size of the amplified products varied from 200bp to 1800bp.  All the primers were found to be polymorphic and produced different percentages of polymorphism.  The average number of fragments per primer was 6 from which 62% were found to be polymorphic fragments. Similarity indices were generated from RAPD data. Since all morphological characteristics of the resistant lines were non significant when compared with control therefore, it can be concluded that the phylogenetic differences among various groups are because of the activation of tolerance level in the highly susceptible Gladiolus cultivar against Fusarium wilt.

Abbreviations: CAMB (Centre for Advanced Molecular Biology), CV (Cultivar), FOG (Fusarium oxysporum f. sp. gladioli) and UPGMA (Unweighted Pair Group Method Arithmetic Average)

¹National Centre of Excellence in Molecular Biology, 87-West Canal Road, Thoakar Niaz Baig, Lahore-53700, Pakistan