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Genetic diversity
in blackgram [Vigna mungo (L.) Hepper] for Randomly Amplified
Polymorphic DNA (RAPD) markers
Abdul Ghafoor,
Tayyaba Sultana1 and zarrin fatima Rizvi2
Abstract:
Thirty seven
pure-lines of blackgram were studied for Randomly Amplified
Polymorphic DNA (RAPD) markers to investigate diversity. Among 53
primers 36 produced polymorphic fragments in blackgram, hence could be
used for investigating genetic diversity. Ninety four markers were
observed from 11 primers and out of these 72% were polymorphic. The
genotypes acquired from Korea, Afghanistan and AVRDC grouped together,
whereas genotypes from Pakistan and India were scattered. The RAPD
markers were found useful for studying genetic diversity but
clustering did not exhibited indication for agronomic performance,
whereas quantitative traits contributed more towards agronomic
performance. Cluster revealed that only a portion of genetic diversity
has been exploited for blackgram improvement that should broaden
involving diverse parents from various clusters.
Institute of Agri
Biotechnology and Genetic Resources (IABGR), National Agricultural
Research Centre (NARC), Park Road, Islamabad,
Pakistan. 1GC University, Faisalabad, Pakistan
2Department
of Agricultural Sciences, Allama Iqbal Open University, Islamabad.
Pakistan
Email:
ghafoor59pk@yahoo.com
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