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Pak. J. Bot., 44(2): 501-506, 2012. |
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Updated: 26-04-12 | ||||
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A Primary Study of High Performance Transgenic Rice Through Maize Ubi-1 Promoter Fusing Selective Maker Gene
Jinjuan Shen1, Pingzhong Cai1,2, FENG QING1, ZhIYong ZHANG AND GUIXUE WANG1*
Abstract:
Based on the
expression vector pBI121, we successfully constructed a plant
overexpression vector of Hspa4 gene fusing with selective maker gene
(hygromycin-resistance gene) driven by the Ubi-1 promoter
(pBI121-Ubi-Hpt-Hspa4, p121UHH). The plant expression vectors p121UHH
and pCAMBIA1301-Ubi-Hspa4 (p1301UH) were transformed into the rice
callus, mediated by Agrobacterium tumefaciens. We screened 17
p121UHH-positive transgenic plants and 15 p1301UH-positive transgenic
plants by the hygromycin-resistance gene. The pick-up rate of the
resistance callus was 51.7% and 42.5%, respectively, and the rate of
regeneration for the resistance callus was 51.2% and 49.1%,
respectively. The result of polymerase chain reaction (PCR)
identification indicated that the pick-up rate of positive transgenic
plants was 51.7% and 42.5% and the total transformation efficiency was
16.5% and 6.2%, and the former was 2.66 times of the later. The results
of the experiment indicate that the possibility of the appearance of
false positive results in the fusing of a plant over-expression vector
with a selective maker gene is much less. 1 Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education; Laboratory of Mechano-developmental Biology, Bioengineering College, Chongqing University, Chongqing 400044, China 2 Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Science, Chengdu 610066, China *Corresponding Author. E-mail: wanggx@cqu.edu.cn. Tel: 86-23-65102508. Fax: 86-23-65102507 |
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