Back to Contents

FREE FULL TEXT PDF

OPTIMIZATION OF THE CULTURE CONDITIONS IN THE RICE (ORYZA SATIVA CV. BASMATI-370)

ROMESA TAHIR¹, MARIA BATOOL¹, NASIR MAHMOOD MINHAS¹, SHAUKAT ALI² SHAHID MASOOD² AND SAIF ULLAH AJMAL^1*

Abstract: The objective of the present study was to develop an efficient callus induction protocol for a fine rice (Oryza sativa L.) variety Basmati-370. Mature grains of this variety were used for callus induction. The Chu’s N6 medium was used as basal medium for callus induction and was supplemented with sucrose, Myo-inositol, Casein, agar (4 g L^-1, 5 g L^-1, 6 g L^-1) and growth hormone 2,4-Dichlorophenoxy acetic acid in different concentrations of 2ppm and 2.5ppm. The genotype responded well for all the three concentrations of agar (4 g L^-1, 5 g L^-1, 6 g L^-1) and two concentrations of 2,4-D (2ppm, 2.5ppm), however, the combination of 4 g L^-1 agar with 2.5ppm 2,4-D gave excellent results with maximum callus induction of 93.42% followed by 4 g L^-1 agar with 2ppm 2,4-D and 5 g L^-1 with 2.5ppm 2,4-D which also gave better results with the observed callus induction of  92.75% and 91.11%, respectively.

¹Department of Plant Breeding and Genetics,PMAS Arid Agriculture University, Rawalpindi, Pakistan.

²Agricultural Biotechnology Programme, Institute of Agricultural Biotechnology & Genetic Resources, National Agricultural Research Center, Islamabad, Pakistan

*Corresponding author: gondaldr@yahoo.com