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A METHOD FOR MID-TERM STORAGE OF EPIMEDIUM PUBESCENS
(BERBERIDACEAE) POLLEN
QIU MEI QUAN AND YUN-XIANG LI*
Abstract:
Due to the different species of Epimedium separated by seasonal
isolation and geography, developing a method for mid-term storage of
E. pubescens is necessary to facilitate germplasm conservation,
interspecific hybridization and identification of hybrid species. In
order to optimize the process of hand-pollination, in this work we have
studied the conservation potential of E. pubescens pollen stored
for approximately 2 months at -20°C, -70°C and -196°C. The optimal
desiccation time of E. pubescens pollen was found to be 210 min.
In vitro pollen germination of fresh pollen was 56.0%, and it was
progressively reduced after conservation at -20°C, -70°C and -196°C
reaching a minimum of 4.2%, 6.0% and 4.4%, respectively after 2 months
of storage. The germination capacity of pollen stored at -20°C and
-196°C was similar to that observed at -70°C, therefore for reasons of
convenience and economy, pollen can be efficiently stored at -20°C.
There were no fruit set differences between the field pollinations
performed with stored pollen and pollinations performed with fresh
pollen. These results indicate that E. pubescens pollen can
effectively stored at subzero temperatures for several weeks to months.
China west normal university, Key Laboratory of Southwest China Wildlife
Resources Conservation, nan chong, si chuan 637002, China
*Corresponding author’s e-mail:
yx_li@263.net;
Tel/Fax: +86 0817 2314311
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