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ASSESSEMENT OF
SOMACLONAL VARIATION IN IN VITRO PROPAGATED CORMELS
OF GLADIOLUS
NOOR-UN-NISA MEMON1*,
MUHAMMAD QASIM2, MUHAMMAD JAFFAR JASKANI2, FAISAL
SAEED AWAN3, AZEEM IQBAL KHAN3, BUSHRA
SADIA3 AND ZAHOOR HUSSAIN4
Abstract:
In vitro
raised cormels of 3.0
to 4.0 mm diameter of 3 commercial grown varieties of gladiolus viz.
Traderhorn, White Friendship and Peter Pears were used to
assess percentage of clonal fidelity with each other and with mother
cormels using Random Amplified Polymorphic DNA (RAPD) and Inter Simple
Sequence Repeat (ISSR) markers.
In order
to regenerate cormels under In vitro conditions, direct
organogenesis was followed using cormel sprout as an explant.
Cormel sprouts obtained by culturing the whole cormels (0.6
g) in a polar position on MS medium supplemented with BAP (4 mg L-1).
Out of the three varieties, White friendship obtained more number
of shoots (22.07). However, statistically similar number of roots was
recorded in White Friendship (22.67) and Peter Pears
(19.60) when MS supplemented with IBA (2 mg L-1). Cormel
production was not affected by any variety of the gladiolus using MS
medium supplemented with IBA (1 mg L-1) and sucrose (7%). The
In vitro produced cormels were assessed for their clonal fidelity
using RAPD and ISSR markers. The RAPD similarity tendencies among In
vitro propagated cormels ranged from 80% to 95% in Traderhorn,
88% to 95% in White Friendship and 80% to 90% in Peter Pears.
However the similarity tendencies between mother and In vitro
propagated cormels, on average were 86% in Traderhorn, 92% in
White Friendship and 83% in Peter Pears. In comparison, ISSR
primers produced higher percentage of similarity matrix than RAPD. The
ISSR cluster analysis for genetic similarity between mother and In
vitro propagated cormels had varied degree of differences detected
90% in Traderhorn, 96% in White Friendship and 85% in
Peter Pears. The genetic differences among In vitro
propagated cormels ranged from 88 to 100% in Traderhorn, 94 to
100% in White Friendship and 82 to 100% in Peter Pears.
1Department
of Horticulture, Sindh Agriculture University Tandojam, Sindh, 70060,
Pakistan
2Institute
of Horticultural Sciences, University of Agriculture Faisalabad-38040,
Pakistan
3Centre
of Agricultural Biochemistry and Biotechnology, University of
Agriculture Faisalbad-38040, Pakistan
4College
of Agriculture D.G. Khan sub-campus University of Agriculture,
Faisalabad, Pakistan
*Corresponding
author:
norimemon@gmail.com
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