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Pak. J. Bot., 44(4): 1209-1213, 2012. |
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Updated: 16-08-12 | ||||
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ANTIBACTERIAL ACTIVITY OF ROOT AND FRUIT EXTRACTS OF LEPTADENIA PYROTECHNICA (ASCLEPIADACEAE) FROM PAKISTAN
MEHMOODA MUNAZIR1, RAHMATULLAH QURESHI1*, MUHAMMAD ARSHAD1 AND MUHAMMAD GULFRAZ2
Abstract: The In vitro antibacterial activity of Leptadenia pyrotechnica roots and fruits extracts was investigated against Staphylococcus epidermidis and S. aureus by using agar-well diffusion assay. Plant samples were collected from Thal desert of Pakistan and eight different solvents viz. n-hexane, chloroform, acetone, ethylacetate, butanol, methanol, ethanol and water were used for the preparation of extracts. S. aureus was found highly susceptible and inhibited by all solvent extracts. Both plant parts effectively inhibited the growth of both the pathogens; however, root extracts showed a little more supremacy in this respect. Methanolic extract of both parts generated the best results by inhibiting growth of both pathogens. The activity was strongly affected by variation in solvents and concentrations of extracts. The antibacterial activity of L. pyrotechnica is reported for the first time.
1Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan 2Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan Corresponding author’s e-mail: rahmatullahq@yahoo.com |
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