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Pak. J. Bot., 44(4): 1357-1362, 2012. |
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Updated: 16-08-12 | ||||
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IN SILICO IDENTIFICATION OF MICRORNAS AND THEIR TARGETS IN FIBER AND OIL PRODUCING PLANT FLAX (LINUM USITATISSIMUM L.)
MUHAMMAD YOUNAS KHAN BAROZAI
Abstract: MicroRNAs (miRNAs) are a class of small, non-coding RNA. They are about 21 nucleotides in length, produced endogenously and negatively regulate gene regulation. The most miRNAs showed conserved nature and this conservation leads to the novel miRNAs discovery applying insilico comparative genomics. Total 26 novel miRNAs from 19 families are identified in Flax (Linum usitatissimum L.). All the miRNA families (miR 156, 159, 162, 166, 167, 171, 172, 319, 394, 397, 408, 783, 1316, 1535, 2108, 3712, 3932, 5014 and 5172) are being reported here for the first time in Flax. All 26 miRNA precursor sequences showed thermodynamically stable structures and the mature miRNAs are appeared in the stem of the stem loop structure. These novel miRNAs in Flax targets transcription factors, retrotransposon, rust resistance genes, cell signaling proteins, maturase K, cellulose synthase, male sterility-related protein and many hypothetical proteins.
Department of Botany, University of Baluchistan, Sariab Road Quetta, Pakistan Corresponding author’s e-mail: barozaikhan@gmail.com |
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