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Pak. J. Bot., 44(4): 1419-1426, 2012. |
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Updated: 16-08-12 | ||||
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INTROGRESSION OF GENETIC MATERIAL FROM ZEA MAYS SSP. MEXICANA INTO CULTIVATED MAIZE WAS FACILITATED BY TISSUE CULTURE
LINGZHI WANG1*, XIAOFENG GU2, XIANPING WANG3, MINGLI QU2, JUNWEN LUAN 2, XIANGQI ZHANG3 AND JUREN ZHANG2*
Abstract: Zea mays ssp. mexicana, a wild relative of cultivated maize (Z. mays ssp. mays), is a useful gene resource for maize breeding. In this study, two populations were generated by conventional breeding scheme (population I) or tissue culture regime (population II), respectively, to introgress genetic material of Z. mays ssp. mexicana into maize. Karyotype analysis showed that the arm ratios of 10 pairs of chromosomes in parent maize Ye515 and derivative lines from 2 different populations with 26% and 38% chromosome variation frequencies, respectively. Alien chromatin was detected in the root tip cells of progeny plants through genomic in situ hybridization (GISH). There were 3.3 chromosomes carrying alien chromatin on average in population I and 6.5 in population II. The hybridization signals were located mainly at the terminal or subterminal regions of the chromosomes and the sizes were notably variant among lines. Based on those results, it is concluded that the introgression of genetic material from Z. mays ssp. mexicana into cultivated maize was facilitated by tissue culture, and subsequently some excellent materials for maize breeding were created.
1School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China 2College of Life Science, Shandong University, Jinan 250100, China 3The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China *Corresponding author’s e-mail: yanyan30186@gmail.com; jrzhang@sdu.edu.cn |
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