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Pak. J. Bot., 44(6): 2009-2014, 2012. |
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Updated: 14-12-12 | ||||
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study of total seed proteins pattern of SESAME (Sesamum indicum L.) LANDRACES via sodium dodecyl sulfate polyacrylamide gel elecrophoresis (SDS-PAGE)
fazal akbar1, nahida yousaf2, M. Ashiq Rabbani2*, Zabta Khan Shinwari1 and M. SHAHID Masood2
Abstract: The sesame (Sesamum indicum L.) germplasm, comprising of 105 accessions was characterized for total seed storage proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The germplasm was collected from diverse agro-ecological regions of Pakistan. To our information, no studies have yet been carried out in Pakistan on the genetic evaluation of sesame genotypes based on total seed protein. Total seed proteins were electrophoretically separated on 12% polyacrylamide gels by standard protocols. A total of 20 polypeptide bands were observed, of which 14 (70%) were polymorphic and 6 (30%) were monomorphic, with molecular weight ranging from 13.5 to 100 kDa. Six bands i.e., 7, 11, 12, 15, 16 and 18 were common in all genotypes. Similarity coefficients varied from 0.50 to 1.00. The dendrogram based on dissimilarity matrix using unweighted pair group method with arithmetic averages (UPGMA) separated all sesame accessions into three main groups i.e., A, B, C, comprising 89, 14 and 2 genotypes, respectively. Overall a low to medium level of genetic variability was observed for SDS-PAGE (single dimension). As SDS-PAGE alone did not reveal high level of genetic variability, hence 2-D gel electrophoresis along with other advanced type DNA markers and more number of sesame accessions from all over the country are recommended for the future genetic evaluation. Our investigation will significantly support the classification, development, genetic evaluation and conservation of sesame germplasm in Pakistan.
1Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 2Institute of Agri-Biotechnology & Genetic Resources, NARC, Islamabad, Pakistan *Corresponding author’s e-mail: rabbani316@yahoo.com |
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