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Pak. J. Bot., 44(6): 2103-2110, 2012. |
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Updated: 14-12-12 | ||||
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GLUCOAMYLASE PRODUCTION FROM ASPERGILLUS NIGER BY USING SOLID STATE FERMENTATION PROCESS
MUHAMMAD IMRAN1, MUHAMMAD JAVAID ASAD1, MUHAMMAD GULFRAZ1, RAHMATULLAH QURESHI2, * HINA GUL1, NAZISH MANZOOR1 AND ARSHAD NAWAZ CHOUDHARY 3
Abstract: The main objective of this study was to produce glucoamylase under optimum conditions and to study the effect of chemical mutagenesis on Aspergillus niger for the production of glucoamylase. The maximum activity of glucoamylase (3.185±0.020 IU/mL/min.) by mutant Aspergillus niger and (2.085±0.021 IU/mL/min.) for wild Aspergillus niger was recorded in the culture filtration after 96 hours of Solid State Fermentation of growth medium with 70% moisture level and in presence of 0.3% yeast extract, 0.4% peptone, and 4 mL Tween-80 at pH 4.8. The maximum fraction value after gel filtration for wild Aspergillus niger was 2.850 IU/mL/min and for mutant Aspergillus niger was 2.980 IU/mL/min. Purification through the SDS PAGE revealed the indication of glucoamylase purification from Aspergillus niger. The high value of Km shows that substrate had great affinity for glucoamylase. Glucoamylase enzyme has many useful applications in food processing industry and fermentation biotechnology.
1Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan 2Department of Botany, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan 2Department of Soil Sciences, Pir Mehr Ali Shah Arid Agriculture University Murree Road Rawalpindi, Pakistan *Corresponding author’s e-mail: rahmatullahq@yahoo.com |
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