|
Pak. J. Bot., 44: 277-284, Special Issue March 2012. |
Back to Contents | ||||
|
|
Updated: 26-04-12 | ||||
|
|
EVALUATION OF TISSUE CULTURE RESPONSES OF PROMISING WHEAT (TRITICUM AESTIVUM L.) CULTIVARS AND DEVELOPMENT OF EFFICIENT REGENERATION SYSTEM
IMRAN MAHMOOD1, ABDUL RAZZAQ1*, ZAHEER-UD-DIN KHAN2, ISHFAQ AHMAD HAFIZ3 AND SHUAIB KALEEM1
Abstract: Efficient callusing and regeneration efficiencies and development of reliable regeneration system are prerequisite for improvement of wheat through recombinant DNA technology. Tissue culture responses of wheat are genotypic, media composition and their interaction dependent. Immature embryos of seven elite wheat cultivars were subjected to in vitro culture processes to select for most appropriate cultivar for tissue culture responses by using auxins and cytokinins one at a time in solidified MS medium. Callus formation and regeneration varied significantly among varieties and among phytohormone concentrations. The interaction of phytohormones and cultivars for callus induction and regeneration was significant and was found to be genotypic and media dependent. Cultivars AS-2002 (92.75%) and GA-2002 (91.25%) produced maximum number of calli on induction media comprising 4 mg/l 2,4-D; while, cv. Chakwal-50 performed best at 6 mg/l 2,4-D (91.75%). The least callusing was witnessed for cv. Sahar-2006 with 2 mg/l of 2,4-D. Induction media comprising 2 mg/l 2,4-D was found inappropriate for all the cultivars except Inqilab-91. None of the cultivars could produce callus at basal MS medium. Independency of regeneration and callusing was observed with maximum regeneration potential of 41.19% for cv. GA-2002 on regeneration medium comprising 1.0 mg/l kinetin. The least regeneration (12.75%) was recorded for cv. Shafaq-2006 on regeneration medium supplemented with 2 mg/l kinetin. A proficient and reliable in vitro regeneration system was successfully established for most tissue culture responsive genotype ‘GA-2002’ and its regeneration was increased from 38.81% to 63.69% by supplementing 0.2 mg/l IAA, 0.5 mg/l Kn and 0.5 mg/l of BAP in regeneration medium. Regeneration frequency of GA-2002 achieved from control (1.0 mg/l kinetin) was improved up to 64.09%. An efficient protocol was developed for rapid screening of tissue culture responsive genotype; and establishment of high frequency regeneration system of wheat rarely reported earlier.
Abbreviations: 2,4-D (2,4-dichlorophenoxyacetic Acid), BAP (6-benzylaminopurine), IAA (Indole-3-acetic acid), 2iP (6-γ-γ-dimethylallylaminopurine), Kn (Kinetin).
1Department of Agronomy, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan 2Department of Botany, GC University, Lahore, Pakistan 3Department of Horticulture, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan *Correspondence: arazzaq57@yahoo.co.in |
||||
|
|
|||||
| Back to Contents |
|
Back to Contents | |||
|
|
|||||