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Pak. J. Bot., 44: 311-314, Special Issue March 2012.

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IN VITRO INDUCTION OF MUTATION IN TOMATO (LYCOPERSICON ESCULENTUM L.) CV. ROMA BY USING CHEMICAL MUTAGENS

MUHAMMAD ISHFAQ¹, IDREES AHMAD NASIR², NASIR MAHMOOD³ AND MUHAMMAD SALEEM¹

Abstract: The aim of present study was to optimize tissue culture conditions for tomato (Lycopersicon esculentum. L) cv. Roma and to induce mutagenesis with different chemical mutagens (Cholchicine, Sodium azide, and Methyl-N-Nitro-N-Nitrosoguanidine abbreviated as MNNG). Tomato seeds were surface sterilized with 0.05 % HgCl₂ and placed on the Petri plates padded with wet cotton, obtained plantlets in 6-7 days. Meristem was isolated from the germinated plants to get pathogen free plants. Media with different hormone compositions were used for callus induction and regeneration. Media having BAP (2.22 µM) proved to be the best callus induction media, while media having IAA (0.75 µM) and zeatin (9.12 µM) was found to be best for regeneration. The calli were treated with different concentrations (0.1 mM- 0.5mM) of the mutagens colchicine, sodium azide and MNNG for ten days. After mutagenic treatment, calli were transferred in regeneration medium with said concentration of hormones and growth parameters of the mutated plants were recorded in comparison to control plants.

¹Department of Botany, University of the Punjab, Lahore-54590.

²National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore.

³Institute of Mycology and Plant Pathology, University of the Punjab, Lahore.

Corresponding author Email: molec.biologist@gmail.com; Tel: 0333-4246280,Fax: 92-42-9231862