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Pak. J. Bot., 44: 329-333, Special Issue March 2012. |
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Updated: 26-04-12 | ||||
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ISOLATION AND MOLECULAR IDENTIFICATION OF A FACULTATIVELY ANAEROBIC BACTERIUM FROM THE HOT SPRING OF AZAD KASHMIR
SANA ZAHOOR*, MUHAMMAD MOHSIN JAVED, MUHAMMAD NAUMAN AFTAB AND IKRAM-UL-HAQ
Abstract: A thermophilic facultatively anaerobic bacterium (TP-1) was isolated from the Tatta Pani hot spring in Azad Kashmir. The isolate had entire and slimy colonies while the cells were small rods and gram-positive. Phylogenetic analysis based on partial 16S rRNA gene sequence comparisons showed high levels of similarity of the TP-1 (> 95%) with Geobacillus pallidus. Optimum temperature and pH for the growth of the strain TP-1 were found to be 65oC and 7.0 respectively. TP-1 gave positive results for ortho Nitrophenyl-β-D-Galactopyranosidase (ONPG) and Gelatin hydrolysis (GEL) while other tests such as Arginine dihydrolases, Lysine decarboxlase, Ornithine decarboxylases, H2S production, Urease, Tryptophan deaminases, Indole production, Acetoin production, Fermentation/oxidation (Glucose, Mannitol, Inositol, Sorbitol, Rhamnose, Sucrose, Melibiose, Amygdalin, Arabinose) and Citrate utilization were negative.
Institute of Industrial Biotechnology, GC University, Lahore, Pakistan *Corresponding author E-mail: sanazahur@yahoo.com; Tel: +92 42 99211634; Fax: +92 42 99211634 |
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