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Pak. J. Bot., 44: 361-364, Special Issue March 2012. |
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Updated: 26-04-12 | ||||
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STUDIES ON THE PRODUCTION OF L-PHENYLACETYLCARBINOL BY CANDIDA UTILIS IN SHAKE FLASK
MARIYAM ABID KHAN, IKRAM-UL-HAQ, MUHAMMAD MOHSIN JAVED*, M.A. QADEER, NAEEM AKHTAR AND SYED ALI IMRAN BOKHARI
Abstract: The present study reveals the production of L-phenylacetylcarbinol (L-PAC) by Candida utilis in 250ml shake flask. Different fermentation media supplemented with various carbon sources were optimized along with different incubation temperatures and inocula to achieve the optimum level of biomass. M1 medium containing (g/L) clarified molasses (30.0 Brix) 250.0, urea 10.0 and MgSO4 20.0 (pH 6.0) gave the optimum biomass level (240x106 cells/ml) after the inoculation of 14 hours old vegetative inoculums (15% v/v containing 240x106 cells/ml) after 6.0 hours at 300C. Biotransformation of benzaldehyde into L-PAC was then carried out with different dose patterns along with supplementation of acetaldehyde. Maximum L-PAC production (4.15 g/L) was observed when 316µl of benzaldehyde was fed in six instalments such as 68, 62, 56, 50, 43, 37 µl in equal interval of 60 minutes at 300C with orbital shaking of 150rpm.
Institute of Industrial Biotechnology, GC University, Lahore, Pakistan *Corresponding author E-mail: mmj_bot@yahoo.com; Ph No. 042-9911634 |
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