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Pak. J. Bot., 44: 377-382, Special Issue March 2012. |
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Updated: 26-04-12 | ||||
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PRODUCTION AND CHARACTERIZATION OF CELLULASES OF ASPERGILLUS NIGER BY USING RICE HUSK AND SAW DUST AS SUBSTRATES
QURAT-UL-AIN1*, S. BAIG2 AND M. SALEEM1
Abstract: The production of cellulases by Aspergillus niger in submerged fermentation with two substrates rice husk and saw dust was compared. The highest cellulase activity was observed at 3rd day in pretreated rice husk media (13.702±0.1) and at 2nd day in pretreated saw dust media (9.683±0.2). The optimum temperature of the enzyme was observed to be around 40șC. It was found that the enzyme activity has a broad pH range between 3-9 and 40% of the original activity was retained after heat treatment at 90șC for 15 min. Maximum cellulase activity obtained when 2.5% substrate was used above this concentration no regular increase in enzyme activity was noticed. Among two lignocellulosic wastes tested in this study rice husk prove to be a good substrate for cellulase enzyme production by the organism Aspergillus niger.
1Department of Botany, University of the Punjab Lahore-54590, Pakistan 2FBRC, PCSIR, Laboratories complex, Lahore-54600, Pakistan *Corresponding author: quratulain_486@yahoo.com |
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