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Pak. J. Bot., 45(1): 269-274, 2013. |
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| Updated: 24-02-13 | |||||
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RANDOM MUTAGENESIS FOR ENHANCED PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS ORYZAE IIB-30
ROHEENA ABDULLAH1, IKRAM-UL-HAQ2, TEHREEMA IFTIKHAR3*, ZAHID ALI BUTT4 AND MANZOOR IQBAL KHATTAK5
Abstract: The current study deals with the isolation of novel mutant of A. oryzae for higher alpha amylase production. The alpha amylase producing A. oryzae strain IIB-30 was subjected to physical (UV) and chemical (Nitrous acid and EMS) mutagenic treatments to enhance the amylolytic potential of parental strain. After mutagenesis isolates were screened both qualitatively and quantitatively. Among these, EMS-20 exhibited highest enzyme activity (280±0.9 U/ml). This mutant showed 2.1 fold increased activity over the parental strain in terms of enzyme production. The result showed the EMS was effective mutagenic agent for stain improvement.
1Department of Biotechnology, Lahore College for Women University, Lahore, Pakistan 2Institute of Industrial Biotechnology, G. C. University, Lahore, Pakistan 3Department of Botany, G.C University, Faisalabad, Pakistan 4Department of Botany Abdul Wali khan university, Mardan,Pakistan 5Chemistry Department, Balochistan University, Quetta, Pakistan *Corresponding author’s e-mail: pakaim2001@yahoo.com |
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