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Pak. J. Bot., 45(4): 1259-1264, 2013.

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GENETIC DIVERSITY IN PEA GERMPLASM USING RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS

SHUJAUL MULK KHAN¹*, HABIB AHMAD², MUHAMMAD NISAR³, SHER ASLAM⁴, INAMULLAH² AND NIAZ ALI¹

Abstract: Selection of the genotypes using plasmid assisted technology provides an efficient and useful tool for elaborating genetic relationships among genotypes. In present study, 48 Pea (Pisum sativum var sativum L.) genotypes obtained from different sources were analyzed through 20 RAPD, DNA markers for assessment of intraspecific DNA variations. Results revealed that significant variations were present in minor bands. Major bands also showed significant diversity. Considerable variations were also recorded in density of some common bands. Maximum and minimum genetic diversity i.e., 80% and 20% was found among 08 and 23 comparisons, respectively from banding profile. These variations can be used further for enhancing variability, a prerequisite for crop breeding. Phylogenetic clustering (through dendrogram analysis) of genotypes revealed that genetic diversity is independent of origin of genotypes. Forty eight genotypes of pea clustered in three main groups A, B and C comprising 23, 5 and 20 genotypes, respectively. Group A1 and C1 included the most distantly related genotypes and hence can be recommended for breeding to obtain genetically diverse segregating populations.

¹Department of Botany, Hazara University Mansehra, Pakistan

²Department of Genetics Hazara University Mansehra, Pakistan

³Department of Botany, University of Malakand, Pakistan

⁴Department of Agriculture Science, University of Haripur, Pakistan

*Corresponding author; e-mail: shuja60@gmail.com