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Pak. J. Bot., 45(6): 2001-2004, 2013.

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AUTOBIOCHROMATOGRAPHY: A TECHNIQUE FOR DETECTION AND ESTIMATION OF THIRAM FROM TREATED SEEDS OF WHEAT

ASHISH KUMAR GUPTA¹, C.R. PRAJAPATI² AND D. SINGH³

Abstract: Wheat seeds treated with different fungicides are qualitatively and quantitatively estimated regularly by different bioassays such as thin layer chromatography, autobiochromatography and spectrophotometry. Autobiochromatography is a highly sensitive method for qualitative and quantitative estimation of small amount of fungitoxic material and useful for detecting a wide range of fungicide using a broad spectrum sensitive fungus. In present investigation double zone of inhibition was observed on chromatoplates in combination of Curvularia lunata and thiram system by autobiochromatography. Plates were spotted with thiram, developed in acetone: chloroform (60:40, v/v) solvent system and autobiochromatography was done with the PDA (5% agar) seeded with the spores of C. lunata. The results of autobiochromatography bioassay revealed that thiram exceptionally produced double zone of inhibition with C. lunata fungus at 100, 250, 500, 1000, 2000 mg/ml concentration. Hence, this information can be used as criterion for detection and identification of thiram extracted from treated seeds.

¹Indian Agricultural Research Institute Regional Station, Pusa (Bihar), India.

²Krishi Vigyan Kendra, Baghpat, S.V.P. Univ. of Agri. and   Tech., Meerut, (U.P.), India

³Krishi Vigyan Kendra, Sitamarhi, (Bihar), India

Corresponding author’s email: ashish.pathology@gmail.com