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Pak. J. Bot., 45(6): 2085-2092, 2013.

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CONSTRUCTION OF MICROSATELLITE LINKAGE MAP AND DETECTION OF SEGREGATION DISTORTION IN INDICA RICE (ORYZA SATIVA L.)

MUHAMMAD ARSHAD JAVED^1*, FAHRUL ZAMAN HUYOP¹, TAKASHIGE ISHII², AZMAN ABD SAMAD¹, TARIQ MAHMOOD³, MUHAMMAD SALEEM HAIDER⁴ AND MUHAMMAD SALEEM⁵

Abstract: High density molecular linkage map is a prerequisite to identify the quantitative traits loci. Construction of linkage maps, using mapping populations derived from two major subspecies: indica and japonica in rice, have been reported. However, limited work was conducted in indica subspecies. Present studies focused to construct a molecular map based on indica × indica (Shaheen Basmati × Pokkali) derived F₂ mapping population. The detected microsatellite polymorphism was only 50.64% between parental cultivars. A total of 107 microsatellite markers were employed to amplify 108 loci, distributed throughout the genome. The marker data of 190 individuals was recorded for map construction. Most of the markers were found co-dominant, where as eight markers exhibited dominance in favor of Shaheen Basmati alleles and four in favor of Pokkali alleles. Detected segregation distortion was 7.47%, which was significantly low than previous studies. MapMaker was used to construct the linkage groups. The map spanned 1753.9 cM (Kosambi function) with microsatellite markers on 12 rice chromosomes and an average distance of 16.2 cM between markers. This map contained a greater and least percentage of markers on linkage group 3 and 12, respectively. Maximum common interval size (MCIS) analysis revealed that present map (indica × indica) covered the rice genome 98% of previous investigation (japonica × indica). Because of diverse background of parental cultivars, low segregation distortion and high genome coverage, revealed that molecular linkage map would be used as marker framework to investigate the genetics of important agronomic traits in indica rice.

¹Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Malyasia

²Lab of Plant Breeding, Graduate school of Agricultural Science, Kobe University, Nada, Kobe  657-8501, Japan

³Plant Breeding Institute, Faculty of Agriculture, The University of Sydney, PMB 11, Camden, NSW 2570, Australia

⁴Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan

⁵Department of Botany, University of the Punjab, Lahore, Pakistan

^*Corresponding author E-mail: majaved@fbb.utm.my; Tel: +60-75-57561; Fax: +60-75558515