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Pak. J. Bot., 45(SI): 41-50, 2013. |
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Updated: 01-02-13 | ||||
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A MODERATELY BORON-TOLERANT CANDIDATUS NOVEL SOIL BACTERIUM LYSINIBACILLUS PAKISTANENSIS SP. NOV. CAND., ISOLATED FROM SOYBEAN (GLYCINE MAX L.) RHIZOSPHERE
RIFAT HAYAT1,2,3*, IFTIKHAR AHMED2*, JAYOUNG PAEK4, MUHAMMAD EHSAN1, 2, MUHAMMAD IQBAL2 AND YOUNG H. CHANG4*
Abstract: A Gram-positive, motile, rod-shaped, endospore-forming and moderately boron (B) tolerant novel candidatus strain, designated as NCCP-54T, was isolated from rhizospheric soil of soybean (Glycine max L.) sampled from the experimental area of Research Farm, PMAS Arid Agriculture University, Rawalpindi, Pakistan. To delineate its taxonomic position, the strain was subject to polyphasic characterization. Cells of the strain NCCP-54T can grow at 10-45○C (optimum at 28○C) at pH ranges of 6.5-9.0 (optimum at pH 7.0) and in 0-6% NaCl (w/v) in tryptic soya agar medium. It can also tolerate 150 mM boric acid in agar medium; however, optimum growth occurs in the absence of boric acid. Based on 16S rRNA gene sequence analysis, strain NCCP-54T showed highest similarity to Lysinibacillus xylanilyticus KCTC13423T (99.1%), Lysinibacillus fusiformis KCTC3454T (98.5%), Lysinibacillus boronitolerans KCTC13709T (98.4%), Lysinibacillus parviboronicapiens KCTC13154T (97.8%), and Lysinibacillus sphaericus KCTC3346T (97.5%) and less than 97% with other closely related taxa. The level of DNA–DNA relatedness between strain NCCP-54T and the type strains of genus Lysinibacillus was <27 %. Strain was also studied chemotaxonomically. Whole-cell peptidoglycan of strain NCCP-54T contained meso-diaminopimelic acid (meso-Dpm) as major diagnostic amino acids instead of lysine-aspartate (Lys-Asp) which is the characteristic of the genus Lysinibacillus. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. MK-7 was the predominant menaquinones. The major cellular fatty acids were iso-C15:0 (30.29%) followed by iso-C16:0 (25.59%) and C16:1w7calcohol (16.24%). The G + C contents of the strain is 37 mol%. The chemotaxonomic characteristics of the candidatus novel strain matched those described for the members of genus Lysinibacillus except diagnostic amino acids contained in peptidoglycans. Phenotypic and phylogenetic analyses thus indicate that strain NCCP-54T may represent a novel candidatus status of species in the genus Lysinibacillus, for which the name Lysinibacillus pakistanensis is proposed. The type strain is NCCP-54T (DSMZ 24784T = KCTC= 13795T). The DDBJ/EMBL/GenBank accession number of the 16S rRNA gene sequence of strain NCCP-54T is AB558495.
1Department of Soil Science & SWC, PMAS Arid Agriculture University, Rawalpindi, 46300, Pakistan 2Plant Biotechnology Program, National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Center (NARC), Park Road, Islamabad-45500, Pakistan 3Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan 4Korean Collection of Type Cultures, Biological Resource Center, KRIBB, 52 Oeundong, Daejeon 305-806, Republic of Korea *Correspondence E-mail: hayat@uaar.edu.pk; iftikharnarc@hotmail.com; yhchang@krib.re.kr |
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