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Pak. J. Bot., 45(S1): 327-332, 2013.

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GENETIC DIVERGENCE AMONG PAKISTANI BREAD WHEAT VARIETIES AND ADVANCED LINES FOR RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

RAHEELA REHMAN^1*, KAUSAR NAWAZ SHAH², M. SHAHID MASOOD¹, M. ARSHAD¹ AND ABDUL GHAFOOR¹

Abstract: A large number of wheat varieties have been developed over the years but very few have been investigated for genetic divergence at molecular level. The present paper explains the genetic diversity for Randomly Amplified Polymorphic DNA (RAPD) markers among wheat varieties as well as advanced lines. Twenty RAPD decamer primers were used to determine the extent of genetic differences among 48 genotypes. Among 20 primers ten were monomorphic and others generated 71 DNA fragments with an average of about 7.1 bands per primer. The primers, viz., OPE-01, OPB-13 and OPB-09 have 17%, 14% and 5.6% share to the total polymorphism among total variation. Forty seven genotypes were amplified with the primer OPA-09 and 11 with OPA-16. Six genotypes NR-346, NR-373, NR-389, NR-383, WSP-148 and WSP-196 were the most diverse from rest of the genotypes for RAPD analysis. The information about genetic similarity and differences will be helpful to avoid any possibility of elite germplasm becoming genetically uniform.

¹National Agricultural Research Centre (NARC), Park Road, Islamabad, PAKISTAN

²Department of Plant Breeding and Genetic PMAS-Arid Agricultural University, Rawalpindi, PAKISTAN

^*Corresponding author’s e-mail: raheelarehman87@gmail.com