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Pak. J. Bot., 45(S1): 339-346, 2013.

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GENETIC FINGERPRINTING OF LOCAL TURMERIC GENOTYPES USING RAPDS

SIFFATULLAH KHAN, SHAMA NAZ, REEMA AND REHAN NAEEM^*

Abstract: Genetic fingerprinting of turmeric (Curcuma longa L.) genotypes collected from 5 different regions of Bannu including Ismail Khel, Kaki, Raakh Sarkar, Michan Khel and Mandan was performed using Randomly Amplified Polymorphic DNA (RAPD). A total of 22 primer were evaluated for genetic diversity studies, of which 3 (OPE-07, OPC-01 and OPA-03) were found suitable. In total these generate 141 fragments, of which 40 fragments were polymorphic with 28.36% of polymorphism. The number of amplification products generated by each primer varied from 6 (OPC-01) to 17 (OPA-03). The polymorphism of turmeric genotypes using OPA-03 (38.3%) was found highest followed by OPE-07 (25.0%) and OPC-01 (21.74%). It was observed that OPA-03 was better to discriminate genotype as compared to other markers. Un-weighed Pair Group Arithmetic Averages (UPGMA) analysis has clustered 50 turmeric genotypes into 6 groups showing their differentiation on the basis of their locality. On the basis of population locality cluster analysis clustered all the collected turmeric populations of Bannu region into two groups including Group I and Group II. Group I include four populations P1, P4, P5 and P2 collected from Ismail Khel, Michen Khel, Mandan and Kaki respectively while Group II was found unique as it comprised of only one population P3 form Raakh Sarkar. Turmeric genotypes evaluated using RAPD in the present study is helpful to identify potential genotypes which give broadness to the germplasm base of turmeric breeding programs.

Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology (KUST), Pakistan

^*Corresponding author’s e-mail: genomics_2003@yahoo.com; Tel.: +92 (0) 922554578. Fax: +92 (0) 922554556