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Pak. J. Bot., 45(SI): 415-422, 2013. |
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Updated: 01-02-13 | ||||
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LEAF RUST RESISTANCE IN SEMI DWARF WHEAT CULTIVARS: A CONSPECTUS OF POST GREEN REVOLUTION PERIOD IN PAKISTAN
GHULAM MUSTAFA*, MUHAMMAD MAQSOOD ALAM, SAMI ULLAH KHAN, MUHAMMAD NAVEED AND ABDUL S. MUMTAZ
Abstract: This study evaluates the genetics of thirty-eight commercial wheat varieties using specific molecular marker for six significant Lr genes (Lr10, Lr13, Lr21, Lr24, Lr26, Lr27 and Lr31) revealing the presence of these genes in 18, 16, 0, 0, 16, 27 and 21 varieties respectively. Thirty one commercial wheat varieties bear more than one Lr genes. To optimize the observations ABI 3730 capillary array method was used for the detection of required product of specific size with sensitivity in single nucleotide polymorphism. The molecular marker with cM distance, less than 1 showed a valuable prediction for effective genes using conventional Gel electrophoresis image. The STS markers showed efficiency to verify four effective genes (Lr10, Lr21, Lr24, and Lr27) in local germplasm with parallel analysis from field trial at Regional Agriculture Institute, Bahawalpur, Pakistan. Specificity of three SSR markers confirmed with sensitive ABI 3730 analysis with fine peak intensities. The data provided showed that the genes (Lr10, Lr13, Lr26, and Lr27-Lr31) are widely distributed in Pakistan varieties while the absence of Lr24 and Lr21, hence provided a motivation to transfer such widely effective genes to enhance resistance through MAS breeding. Findings showed that the marker assisted selection employing sensitive ABI 3730 analysis of distributed Lr genes in Pakistani wheat will help to establish gene pyramiding against leaf rust races and hence a way forward to integrate effective genes in future wheat varieties. The reliability of STS and specific SSR marker under diverse genetic background will also be a futuristic approach.
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan. *Corresponding author’s e-mail: mustafascholar@yahoo.com |
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