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Pak. J. Bot., 45(S1): 423-431, 2013.

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GENETIC DIVERSITY IN BASMATI AND NON-BASMATI RICE VARIETIES BASED ON MICROSATELLITE MARKERS

SHAHID MASOOD SHAH^*, SHAHZAD AAMIR NAVEED AND MUHAMMAD ARIF

Abstract: Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. The purpose of this study was to evaluate the genetic diversity within a diverse collection of rice (Oryza sativa L.) accessions and to determine differences in the patterns of diversity within the aromatic and non-aromatic rice varieties. Forty rice accessions were evaluated by means of 24 microsatellite markers distributed over the whole rice genome. A total of 66 alleles were detected at 24 SSR loci, and the number of alleles per marker ranged from 2 to 4, with an average of 2.75. Polymorphism information content (PIC) value ranged from 0.0476 (RM315) to 0.5993 (RM252), with an average of 0.3785 per marker. The average genic diversity over all SSR loci for the 40 genotypes was 0.4477, ranging from 0.0488 to 0.6638. Major allele frequency ranged from 0.4250 (RM252) to 0.9750 (RM315), with an average of 0.6472. The dendrogram based on the cluster analysis by microsatellite polymorphism, grouped 40 rice cultivars into three groups effectively differentiating basmati cultivars from non-basmati cultivars. These results could be useful for monitoring purity, genotype identification and for plant variety protection.

National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan

^*Corresponding author’s e-mail: smasood.nibge@gmail.com