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Pak. J. Bot., 45(S1): 529-534, 2013.

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EFFECT OF ACC DEAMINASE BACTERIA ON TOMATO PLANTS CONTAINING AZO DYE WASTEWATER

SHEREEN KHALIQ¹, AZEEM KHALID^1*, BEENISH SABA¹, SHAHID MAHMOOD¹, MUHAMMAD T. SIDDIQUE² AND IRFAN AZIZ³

Abstract: The effect of plant growth promoting rhizobacteria (PGPR) on seedling germination, plant growth and biomass yield of tomato plant in presence of dye wastewater irrigation was evaluated in 2 experiments (lab scale and green house). Previously isolated strain Pseudomonas fluorescens (Q14) with ACC deaminase activity was used to check its effect to remedify dye contaminated water impact on plants. The maximum biomass production was obtained on application of 600 mg/L dye with selected PGPR as compared to control water. The dye concentration beyond 600 mg/L showed significantly repressed growth of plants. In plant biomass analysis root and shoot biomass were measured while in plant growth root, shoot length in both experiments was compared. It was also observed that the inoculation with plant growth promoting bacteria carrying ACC deaminase activity promote root and shoot growth as compared to un-inoculated plants at different Reactive Black B concentrations. Maximum shoot (46 cm) and root length (35cm) was observed at 600 mg/L of Reactive Black B with inoculation, which clearly implies the importance of industrial wastewater for crop and vegetable grown under water stress conditions. These findings suggest that PGPR with ACC deaminase activity could be implied for increasing biomass production of wheat and maize irrigated with dye containing wastewater released from textile industries. 

¹Department of Environmental Sciences, PMAS Arid Agriculture University Rawalpindi, Pakistan

²Department of Soil Science & SWC, PMAS Arid Agriculture University Rawalpindi 46300, Pakistan

³Department of Agronomy, PMAS Arid Agriculture University Rawalpindi 46300, Pakistan

^*Corresponding author’s e-mail: azeemuaf@yahoo.com