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Updated: 15-01-14 | ||||
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ROLE OF ABSCISIC ACID (ABA) IN MODULATING THE RESPONSES OF TWO APPLE ROOTSTOCKS TO DROUGHT STRESS
LINSEN ZHANG1, XUEWEI LI 1, LIXIN ZHANG2*, BINGZHI LI 1, MINGYU HAN 1, FUTING LIU1, PENG ZHENG 2 AND ASHOK K. ALVA 3
Abstract: Drought stress is considered as the main limiting factor for apple (Malus domestica L.) production in some semi-arid areas of China. In this study, we investigated the modulation role of abscisic acid (ABA) and fluridone (ABA synthesis inhibitor) on water relations and antioxidant enzyme system in 2-year-old seedlings of two apple rootstocks i.e. Malus sieversii (Ledeb.) Roem. (MS) and Malus hupehensis (Pamp.) Rehd. (MH). Drought stress induced ion leakage, accumulation of malondiadehyde (MDA) and decreases in leaf water potential and relative water content (RWC) in both rootstocks, which were significantly alleviated by exogenous ABA application. Drought stress also induced markedly increases in endogenous ABA content and activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR), and glutathione reductase (GR), to a greater magnitude in MS as compared to MH rootstock. Concentration of 100μmol/L and 50μmol/L ABA had the most positive effects on drought-stressed rootstocks of MS and MH, respectively. Spraying optimum exogenous ABA contributed to enhancement in most of the above antioxidant enzymes activities but reduction in content of MDA and maintained the appropriate leaf water potential and RWC in both rootstocks. Pretreatment with fluridone aggravated ion leakage and the accumulation of MDA in two apple rootstocks under drought stress, which was overcome by exogenous ABA application to some extent. In conclusion, the endogenous ABA was probably involved in the regulation of two apple rootstocks in responses to drought stress.
1College of Horticulture, Northwest A&F University, 712100, Yangling, Shaanxi, P.R. China 2College of Life Sciences, Northwest A&F University, 712100, Yangling, Shaanxi, P.R. China 3USDA-ARS-Vegetable and Forage Crops Research Unit, 24106-N. Bunn Road, Prosser WA 99350, USA *Corresponding author’s e-mail: zhanglixin@nwsuaf.edu.cn |
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