Pak. J. Bot., 46(1): 279-288, 2014. |
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Updated: 15-01-14 | ||||
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EFFECT OF SUCROSE, BENZYLAMINOPURINE AND CULTURE CONDITION ON IN VITRO PROPAGATION OF CURCUMA XANTHORRHIZA ROXB. AND ZINGIBER AROMATICUM VAL.
MELATI YULIA KUSUMASTUTI1, ARVIND BHATT3*, GUNAWAN INDRAYANTO2 AND CHAN LAI KENG1
Abstract: Curcuma xanthorrhiza
and
Zingiber aromaticum,
are important medicinal species of the Zingiberaceae family. They are used in traditional medicine known as Jamu. This
study reports on the presence of sucrose and BA (benzylaminopurine) supplements in the culture medium and also the effect of
varying culture condition on
In vitro
propagation of these two species. Murashige & Skoog (MS) medium supplemented with
5 mg L-1 BA and 30 g L-1 sucrose, incubated under total darkness, induced the highest number of multiple shoots
in C. xanthorrhiza
while the
Z. aromaticum
produced the highest number of multiple shoot in the same medium incubated
under continuous light. The increase in sucrose concentration reduced shoot height in both species. Rooting of
micro-shoots was not affected by the different culture treatments. The acclimatized plantlets of
C. xanthorrhiza
and
Z. aromaticum
were normal with 83.3-100%
percentage when transferred to the outside environment. 1School of Biological Science, Universiti Sains Malaysia, 11800 Penang 2Faculty of Pharmacy, Airlangga Univerity, 60286 Surabaya 3School of Life Sciences (Biology Building), University of KwaZullu – Natal, Durban South Africa *Corresponding author’s e-mail: drbhatt79@rediffmail.com |
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