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Pak. J. Bot., 46(4): 1531-1535, 2014.

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SCREENING OF SUGARCANE SOMACLONES OF VARIETY BL4 FOR AGRONOMIC CHARACTERISTICS

SABOOHI RAZA*^1,2, SYEDA QAMARUNNISA¹,  ISHRAT JAMIL¹,  BEENA NAQVI³, ABID AZHAR¹ AND  JAVED A. QURESHI¹

Abstract: Considering the environmental conditions of Pakistan where sugarcane breeding is constrained due to non viable fuzz (seeds) production.  Somaclonal variation could prove to be a useful tool to overcome the difficulties in cane breeding. In the present study, 324 sugarcane somaclones regenerated from immature leaf roll callus of sugarcane variety BL4 were evaluated for their yield and yield contributing characters and the quality traits of cane. The field trial of somaclones showed variation in 160 somaclones from the mother plant in at least one character observed. Most of the somaclones showed variation in weight of stalks per plant; however, only twenty four out of 89 clones showed increase in the weight of the stalks per clump. The second highly variable trait was the number of stalks, 88 plants showed either increase or decrease in the number of stalks. It is noteworthy that the sucrose accumulation was not increased in any of the somaclones. Twenty one somaclones were selected for their increased yield potential. The comparative performance of these selected clones revealed that clones ‘K-250, K-265, K-251, K-109, K-106, K-300 and K-315 gave better sugar yield /plant as compared to BL4. Maximum sugar yield/plant was observed in Clone ‘K-250’ (2.5 Kg) followed by K-265 (2.44 Kg), whereas the average sugar yield of BL4 was 1.2 Kg/plant.

¹The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Pakistan.

²Nuclear Institute of Agriculture (NIA), TandoJam, Pakistan.

³Pakistan Council of Scientific and Industrial Research (PCSIR), Karachi, Pakistan

*Correspondence e-mail: razasaboohi@yahoo.com; Tel #  092213013559827