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ALLEVIATION OF ADVERSE IMPACT OF SALINITY ON FABA BEAN (VICIA FABA L.) BY ARBUSCULAR MYCORRHIZAL FUNGI
 

 HASHEM ABEER1*, ABD_ALLAH, E.F.2, ALQARAWI, A.A.2, EL-DIDAMONY, G.3, ALWHIBI MONA, S.1, EGAMBERDIEVA D.4 AND AHMAD, P.5
 

Abstract: The present study was conducted to assess the effect of different concentrations of sodium chloride (NaCl) in presence and absence of AMF on growth, physio-biochemical and enzymatic activity in faba bean (Vicia faba). Different concentrations of NaCl showed reduction in growth and yield parameters, which indicates the deleterious effects of salinity on the plant. The total spore count and colonization by arbuscular mycorrhizal fungi (AMF) is also decreasing at higher concentrations of NaCl. Application of AMF mitigates the effect of NaCl stress and improved the growth and yield in the present study. NaCl also decreased the nodulation as well as nodule activity and pigments content, however the supplementation of by AMF to plants treated with sodium chloride showed enhancement in nodule activity and pigment content. Polyamines (Putresciene, Spermidine, Spermine), acid and alkaline phosphates increased with increasing concentration of sodium chloride and application of by AMF showed further increase in the above phytoconstituents, proving the protective role of these phytoconstituents against salt stress. Salinity stress is responsible for the generation of reactive oxygen species, which lead to the membrane damage through lipid peroxidation in the present study. Maximum lipid peroxidation was observed at higher concentration of sodium chloride and AMF treatment minimized the effect of salinity on lipid peroxidation. To combat with the reactive oxygen species, plants upregulate the enzymatic antioxidants like superoxide dismutase, catalase, peroxidase and ascorbate peroxidase. As the concentration of sodium chloride increases the enzyme activity also increases and further increase was observed with supplementation of AMF to salt treated plants. Arbuscular mycorrhizal fungi also restores the potassium and calcium contents and maintain their ratio that was hampered with increasing concentration of sodium chloride in the present study. In conclusion, application of AMF could alleviate the negative effect of sodium chloride and can be used as a suitable approach for the salt affected soils.

Key words: Salt stress; Vicia faba; growth, pigments; nodulation; polyamines; lipid peroxidation; enzymatic activity; elemental uptake.

¹Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia

²Department of Plant Production, Faculty of Food & Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia

³Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt

⁴Faculty of Biology and Soil Sciences, National University of Uzbekistan, 100174 Tashkent, Uzbekistan

⁵Department of Botany, S.P. College, Srinagar, Jammu and Kashmir, 190001, India

*Corresponding author’s e-mail: habeer@ksu.edu.sa