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Pak. J. Bot., 44(2): 477-484, 2015.

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SYSTEMATIC VALIDATION OF MEDICINALLY IMPORTANT GENUS EPIMEDIUM SPECIES BASED ON MICROSATELLITE MARKERS

ZUBAIDA YOUSAF^1*, WEIMING HU², YANJUN ZHANG², SHAOHUA ZENG³AND YING WANG³

Abstract: Epimedium is taxonomically complicated in terms of identification and availability of limited phenotypical markers. Therefore in the present study, 36 SSR primers markers were used for 44 individuals belonging to 13 medicinal species of the Epimedium genus and one out group species Vancouveria hexandra W. J. Hooker to resolve their existing taxonomic problems. A total of 164 alleles by genomic SSR were detected. The markers were presented between 2-10 alleles per locus. Jacard index cluster analysis revealed two main and four subclusters. Principle component analysis indicated that genetic variability is analogous to geographical variability. It has been concluded that medicinally important species of the genus Epimedium possesses sufficient genetic variation for effective resolution of the existing taxonomic problems in combination of morphological markers. .

Key words: Epimedium, Genomic SSR/EST SSR markers, Genetic diversity, Systematics.

¹Department of Botany, Lahore College for Women University, Jail Road Lahore, Pakistan 03334272550,

²Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, the Chinese Academy of Sciences, Wuhan, Hubei, China

³Key Laboratory of Plant Resources Conservation and Sustainable utilization, South China Botanical Garden, Chinese Academy of Sciences, Gaungzhou 510650, PR China.e-mail: yingwang@wbgcas.cn

^*Corresponding e-mail:  zubaida_yousaf@yahoo.com