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Pak. J. Bot., 47(3): 937-950, 2015. |
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Updated: 23-06-15 | ||||
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A SYSTEMATIC REVISION ON CARALLUMA SPECIES OF SAUDI ARABIA BASED ON KARYOLOGICAL AND MOLECULAR DATA
S.M. SHARAWY1,2*, E.A. KAMEL3,5 , E.A.K. KARAKISH1,4 AND M.H.A. LOUTFY5
Abstract: A systematic revision of 13 species of the genus Caralluma (Apocynaceae – Asclepiadoideae – Ceropegieae) from the flora of Saudi Arabia based on the evaluation of karyological and molecular data was carried out. Karyotype criteria and polyploid variations were discussed. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Random Amplified Polymorphic DNA (RAPD-PCR) techniques were also used in this study to distinguish these species. Karyotype features of the studied species and molecular data were used to reassess the relationships of the 13 species of Caralluma in the light of the current systems of classification.
Key words: Ceropegieae, Karyotype analysis, SDS-PAGE, Protein analysis, DNA (RAPD-PCR), Systematic relationships.
1Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt 2Biology Department, Faculty of Science, Hail University, Hail, Saudi Arabia 3Biology Department, University College, Umm Al-Qura University, Makkah, Saudi Arabia 4Biology Department, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia 5Biological Sciences & Geology Department, Faculty of Education, Ain Shams University, Cairo, Egypt *Corresponding author e-mail: sherifsharaawy@yahoo.com |
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