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Pak. J. Bot., 47(3): 1087-1093, 2015. |
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Updated: 23-06-15 | ||||
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RELATIONSHIP OF ASCORBIC ACID METABOLISM WITH THE CYTOPLASMIC MALE STERILITY IN PEPPER (CAPSICUM ANNUUM L.)
JINFEN WEN1†, MINGHUA DENG1,2†, JINLONG HUO2†, HAISHAN ZHU2, XIONGZE DAI3, ZHUQING ZHANG3, JUNHENG LV2 AND XUEXIAO ZOU3*
Abstract: To elucidate the metabolic mechanism of Ascorbic Acid (ASA) in the CMS-pepper anthers, the metabolism changes in the reactive oxygen species (ROS) contents, antioxidants contents and ROS scavenging enzymatic activities were investigated in the anther mitochondria of CMS-9704A and maintainer-9704B. At the abortion stage, anthers of CMS-9704A had higher contents of ROS than those of the maintainer. Simultaneously, there were lower contents of ASA and glutathione (GSH) in stage 2 and 3, and the lower activities of ascorbate peroxidase (APX) and glutathione perodixase (GPX) in stage 3 in scavenging ROS in the anthers of the CMS line than maintainer. The expression level of APX and GPX in stage 3 in anthers of CMS-9704A was obviously inhibited when ROS produced with a great deal during anther stage; however the gene expression kept normal in the maintainer. Excessive accumulation of ROS, significant reduction activities and gene expression level of ROS-scavenging enzyme were coinstantaneous with CMS.
Key words: Capsicum annuum L., Mitochondrion, Cytoplasmic male sterility, Metabolism of ascorbic acid, Gene expression.
1Kunming University of Science and Technology, Kunming 650224, China 2Yunnan Agricultural University, Kunming 650201, China 3Hunan Academy of Agricultural Science, Changsha 410125, China *Corresponding author’s e-mail: pepper_breed@163.com or Zou_xuexiao@163.com †Both these authors contributed equally to this work and share the first authorship
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