Back to Contents

Pak. J. Bot., 47(3): 1095-1102, 2015.

FREE FULL TEXT PDF

DETERMINATION OF GENOMIC DIVERSITY WITHIN MUTANT LEMON (CITRUS LIMON L.) AND MANDARIN (CITRUS RETICULATA) USING MOLECULAR MARKERS

ILKNUR POLAT^*, ERTUGRUL TURGUTOGLU AND SENAY KURT

Abstract: Mutation breeding is considered to be successful in order to obtain new cultivars and expanding the genetic source of new varieties of lemon and mandarin in Citrus. In this study, different doses of gamma radiation (⁶⁰Co) have been used to generate novel mutants of the common variety Yerli Yuvarlak lemon and Yerli mandarin. The differences within the mutants were examined using simple sequence repeats (SSR) and sequence related amplified polymorphism (SRAP) markers. A total of 26 SSR primers and 28 SRAP primer combinations were screened for linkage to the different traits. Six SSR primers were showed polymorphism and closely linked to shoot tip color in lemon. Whereas, all of SRAP primer combinations were not produce any scorable polymorphic bands in both Yerli Yuvarlak lemon and Yerli mandarin. In this study, SSR and SRAP molecular markers were used together for first time to determination the genetic differences in citrus mutation breeding in the world.

Key words: Lemon; Mandarin; Mutation breeding; Shoot tip color; SSR; SRAP.

Bati Akdeniz Agricultural Research Institute, 07100 Antalya-Turkey

^*Corresponding author’s e-mail: i_polat@hotmail.com