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Updated: 23-06-15 | ||||
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Relationships evaluation on six herbal species (Curcuma) by DNA barcoding
JiaBin Deng 1 , Jia Liu1, Khawaja Shafique Ahmad3, ChunBang Ding1, Li Zhang1, YongHong Zhou2 aND RuiWu Yang1*
Abstract: Three chloroplast regions, rbcL, psbA-trnH and petA-psbJ were applied to assess the genetic relationships among six Curcuma medicinal species, which are difficult to distinguish from morphology. The Maximum Parsimony tree was conducted by Kimura 2-parameter model with MEGA 4. The genetic relationships were linked with geographical distributions among these six species; Curcuma sichuanensis is a mutation species of Curcuma longa, Curcuma sichuanensis couldn’t be defined as a single species, and Curcuma chuanhuangjiang is an individual species.
Key words: Curcuma, DNA barcoding, Genetic rbcL, psbA-trnH, petA-psbJ.
1 College of Life Science, Sichuan Agricultural University, 625014, Yaan, China 2Triticeae Research Institute, Sichuan Agricultural University, 611830, Wenjiang, China 3Department of Botany, University of Agriculture, Faisalabad, 38040, Pakistan *Corresponding author’s e-mail: yrwu@sicau.edu.cn |
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